Seminar International Probiotic Conference Juni 13-17th 2010, Slovakia

The Growth and survival of Lactic Acid Bacteria was Isolated from By-product Virgin Coconut Oil as Probiotic Candidate for Poultry

Husmaini¹; M.H. Abbas¹; E.Purwati¹ and A. Yuniza¹

¹Faculty of Animal Science, Andalas University, Limau Manis Campus,

West Sumatera, Indonesia

E-mail: husmaini_tando@yahoo.com

ABSTRACT

The present study was aimed characterizing LABs from by-product of VCO processing and to study probiotic properties candidate in-vitro condition for use in poultry. Forty eight LABs were isolated and four of them were selected. The growth and survival rate of LABs (Lh1, Lh2, Lh3 and Lh4) in both 37⁰C and 42⁰C temperature, tolerance of LABs to acidic pH was determined by growing bacteria in acidic MRS broth. MRS broth was poured’ pH 2.0, 5.7 and 7.0 was adjusted with 1N HCl and 0.5N NaOH. Bacterial survival was evaluated after 15, 30, 60, 90 and 120 and 480 minutes of incubation. Tolerance of LABs to stimulated gastric transit, each isolated bacterial cultures were mixed with 3 ml of stimulated gastric juice and 1 ml of phosphate buffer saline.. Bacterial survival was evaluated after 30, 60, 90, 120 and 480 minutes of incubation. Antibiotic sensitivity test was performed by Muller Hinton's agar. The radial width of the zones outside the antibiotic discs was measured in mm. The starting inoculums was arrange which optical density (OD)₅₈₀nm at absorbance 0.05 was used to measure growth. The results showed that the Lh4 proved to be highly resistant and survive in comparison with the other LABs. At 42⁰C temperature, Lh4 and Lh1 are growth as well as at 37⁰C temperature incubation. The growth of all LABs at pH, 5.7 and 7.0 showed well growth similarly, but at  pH 2 only Lh4 grown whereas Lh1,Lh2 and Lh3 couldn’t growth.. All of LAB can grow at stimulated gastric transit. Lh4 was no sensitive to all antibiotic respectively (clear zones: 0,33mm) but the other LABs were sensitive (clear zones:5 – 12 mm). The conclusion is the variety responses of LAB in-vitro condition were probably due to differences in the ability of LAB to grow and survive in gastro intestinal tract. While Lh4 showed grown and most survive at all of in-vitro condition. Thereby only Lh4 use as candidate probiotic for poultry.

Key words:  lactic acid bacteria, probiotic, survive, growth

Seminar Permi 2010

Effect of Lactococcus Plantarum Was Isolated from Byproduct Virgin Coconut Oil Processing as Probiotic on Intestinal Microflora of Broiler

HUSMAINI^1*, MUHAMMAD HAFIL ABBAS¹, ENDANG PURWATI¹,  AHADIYAH YUNIZA¹ and WAZIR¹

Departemen of Animal sains, Animal science Faculty, Andalas University, Padang West Sumatera Indonesia

A study was conducted to determine the effect of multiple dose levels of Lactococcus plantarum culture on microflora intestine (duodenum and ileum) of broilers. This study used 120 broilers were divided into 5 treatment groups in a random. The treatments were given the dosage of Lactococcus plantarum culture were 0.0ml, 0.5ml, 1.0ml, 1.5 ml per bird and the commercial probiotics as a comparison. The Lactococcus plantarum was isolated from byproducts of Virgin Coconut Oil processing. Lactococcus plantarum culture is given in a liquid with optical density (OD₅₈₀ = 0.5) as oral (1 ml culture is identical to ± 1.3 x 10⁸ cfu Lactococcus plantarum). The chicken was slaughtered at 6 weeks age, For calculated the number of lactic acid bacteria, is used MRS Agar (Merck’s) + 0.05% CaCO3, whereas for calculated both Salmonella and E. coli are used Chrome Agar. Microbiological examination of duodenum and ileal content indicated an obvious was increased total count Lactic acid bacteria and decreased both were Escherichia coli and Salmonella sp at various dose level of Lactococcus plantarum culture. In this study, no detected Salmonella sp in both duodenum and ileum broiler on dilution 10^-2, when was given 1.5 ml culture of Lactococcus plantarum per bird every two weeks.

Keywords : Lactococcus plantarum, byproduct of VCO processing, Microflora , intestinal and  broiler