Seminar Permi 2010

Effect of Lactococcus Plantarum Was Isolated from Byproduct Virgin Coconut Oil Processing as Probiotic on Intestinal Microflora of Broiler

HUSMAINI^1*, MUHAMMAD HAFIL ABBAS¹, ENDANG PURWATI¹,  AHADIYAH YUNIZA¹ and WAZIR¹

Departemen of Animal sains, Animal science Faculty, Andalas University, Padang West Sumatera Indonesia

A study was conducted to determine the effect of multiple dose levels of Lactococcus plantarum culture on microflora intestine (duodenum and ileum) of broilers. This study used 120 broilers were divided into 5 treatment groups in a random. The treatments were given the dosage of Lactococcus plantarum culture were 0.0ml, 0.5ml, 1.0ml, 1.5 ml per bird and the commercial probiotics as a comparison. The Lactococcus plantarum was isolated from byproducts of Virgin Coconut Oil processing. Lactococcus plantarum culture is given in a liquid with optical density (OD₅₈₀ = 0.5) as oral (1 ml culture is identical to ± 1.3 x 10⁸ cfu Lactococcus plantarum). The chicken was slaughtered at 6 weeks age, For calculated the number of lactic acid bacteria, is used MRS Agar (Merck’s) + 0.05% CaCO3, whereas for calculated both Salmonella and E. coli are used Chrome Agar. Microbiological examination of duodenum and ileal content indicated an obvious was increased total count Lactic acid bacteria and decreased both were Escherichia coli and Salmonella sp at various dose level of Lactococcus plantarum culture. In this study, no detected Salmonella sp in both duodenum and ileum broiler on dilution 10^-2, when was given 1.5 ml culture of Lactococcus plantarum per bird every two weeks.

Keywords : Lactococcus plantarum, byproduct of VCO processing, Microflora , intestinal and  broiler